updating changes to PlatE retroviral production protocol and spinfection to reflect new option to concentrate

Author: m-caste

docs/protocols/tc/virus/platE_prod.rst

@@ -59,7 +59,14 @@ Day Two (transfect Plat-Es):
 
 Day Three (Plat-E media change + seed mouse cells):
 
+
 1.	Change with 1.25 mLs fresh media (:ref:`DMEM/HEPES + 10% FBS <HEPES>`) after 24 hours. Note: NBW transfects ~4pm and media changess ~10am next day to minimize PEI cytotoxicity.
+
+.. note::
+
+    Instead of infecting MEFs with unconcentrated retrovirus for two days, you can also media collect for 2 days and concentrate, same as the :ref:`HEK293T protocol <virusProd>`.
+    BAD and MC resuspend concentrated retrovirus in 100 uL media per well of a 6 well, and :ref:`spinfect <spinfection>` MEFs for 1 day with 2 uL concentrated virus.
+
 2.	Seed mouse cells.
 
     i.  Coat wells in 0.1% gelatin for approx. ~10 min.

docs/protocols/tc/virus/virus_infection.rst

@@ -104,10 +104,9 @@ The combined protocol of transduction plus spinning is dubbed "spinfection."
 
 .. important:: Cover the centrifuge buckets with the plate spinner tray caps.
 
-2. Centrifuge the plate at 1500 x g for 90 min at 32ºC.
+2. Centrifuge the plate at 1500 x g for 30 min at 32ºC.
 
 .. note:: The centrifuge won't heat to 32ºC before the spin, but it will warm up during.
 
-.. note:: Spins shorter than 90 min may also be effective.
 
-3. After the spin is complete, transfer the plate to the 37ºC incubator. Continue as normal with the 1 dpi media change and subsequent treatments, readouts, etc.
+1. After the spin is complete, transfer the plate to the 37ºC incubator. Continue as normal with the 1 dpi media change and subsequent treatments, readouts, etc.